Determination of “Net Carbohydrates” Using High-Performance Anion Exchange Chromatography

Analysis of Sialic Acids Using High-Performance Anion-Exchange Chromatography

INTRODUCTION Sialic acids comprise a large family of Nand O-substituted neuraminic acids. The amino group of neuraminic acid is linked to either an N-acetyl or N-glycolyl group, which yields N-acetylneuraminic acid (Neu5Ac) and N-glycolylneuraminic acid (Neu5Gc), respectively. The hydroxyls of both sialic acids are often modified. The biosynthesis, function, and occurrence of sialic acids have ...

Analysis of Carbohydrates by High-Performance Anion-Exchange Chromatography with Pulsed Amperometric Detection (HPAE-PAD)

Anion-Exchange Chromatography I. Mechanism of Separation Although anion-exchange chromatography has been used extensively to analyze acidic carbohydrates and glycopeptides, it has not been commonly used for analysis of neutral sugars. However, examination of the pKa values of the neutral monosaccharides listed in Table 1 shows that carbohydrates are in fact weak acids. At high pH, they are at l...

Effect of temperature on the retention of amino acids and carbohydrates in high-performance anion-exchange chromatography.

Effect of temperature on the retention of amino acids and carbohydrates in high-performance anion-exchange chromatography (HPAEC) was investigated. Amino acids retention exhibited both exothermic and endothermic behavior. Significant selectivity variations of some weakly retained amino acids were achieved through changing temperature. However, temperature was almost ineffective at changing the ...

Rapid Determination of Lactose and Lactulose in Dairy Products Using High Performance Anion-Exchange Chromatography with Pulsed Amperometric Detection

Determination of Sucralose in Reduced- Carbohydrate Colas using High Performance Anion-Exchange Chromatography with Pulsed Amperometric Detection

INTRODUCTION Sucralose (trichlorogalactosucrose or 1,6-dichloro1,6-dideoxy-b-D-fructofuranosyl-4-deoxy-a-D-galactopyranoside) is a non-nutritive sweetener used to manufacture diabetic and dietetic foods and beverages. Detection of sucralose and other carbohydrates is challenging because they lack a strong chromophore and, therefore, cannot be detected at low concentrations with UV detection. Fu...